1T15

Crystal Structure of the Brca1 BRCT Domains in Complex with the Phosphorylated Interacting Region from Bach1 Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-01-17
Detector	RIGAKU RAXIS II
Spacegroup name	P 32 2 1
Unit cell lengths	65.837, 65.837, 93.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.850
Rwork	0.206
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.930
High resolution limit [Å]	1.850	1.850
Number of reflections	19219
Completeness [%]	93.9	76.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG 8000, Ammonium Sulphate, MES, pH 6.5, Microbatch, temperature 291K