1T0O
The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.834, 58.313, 153.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 1.960 |
| R-factor | 0.15686 |
| Rwork | 0.151 |
| R-free | 0.20491 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1szn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.594 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 28741 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 63.6 | 64 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | EG 6000, potassium phosphate, sodium phosphate, D-galactose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
