1T0I
YLR011wp, a Saccharomyces cerevisiae NA(D)PH-dependent FMN reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979678 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.801, 109.957, 49.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.20175 |
| Rwork | 0.199 |
| R-free | 0.25436 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.743 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 27164 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 96.9 | 95.5 |
| Redundancy | 4.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 10% PEG 3000, 30% PEG 400, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
