1T0F

Crystal Structure of the TnsA/TnsC(504-555) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	CCD
Collection date	2003-03-11
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	156.032, 70.112, 89.681
Unit cell angles	90.00, 123.61, 90.00

Refinement procedure

Resolution	42.410 - 1.850
R-factor	0.211
Rwork	0.211
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f1z
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.850	1.850
Rmerge	0.078	0.386
Number of reflections	66363
<I/σ(I)>	9.3	2.37
Completeness [%]	97.9	99.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	malonate, lithium sulfate, 2,4 methylpentanediol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K