1T0B
Structure of ThuA-like protein from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-06-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.28136 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.542, 74.603, 123.518 |
| Unit cell angles | 90.84, 97.78, 118.69 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.14484 |
| Rwork | 0.145 |
| R-free | 0.17536 |
| Structure solution method | Se-SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 207543 |
| Completeness [%] | 85.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | VAPOR DIFFUSION, HANGING DROP, temperature 273K |
