1T06
1.9 A Crystal Structure of a Protein of Unknown Function from Bacillus cereus ATCC 14579
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-30 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.959, 89.112, 121.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.437 |
| Number of reflections | 72674 | |
| <I/σ(I)> | 18.18 | 2.12 |
| Completeness [%] | 95.9 | 77 |
| Redundancy | 6.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1.2M NH4SO4, 20% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
