1SZR
A Dimer interface mutant of ornithine decarboxylase reveals structure of gem diamine intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 124 |
| Detector technology | CCD |
| Collection date | 2002-04-01 |
| Detector | SBC-2 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.468, 152.444, 155.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.150 |
| Rwork | 0.244 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2TOD with ligands and Lys-294 removed |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.074 | 0.558 |
| Number of reflections | 84756 | |
| <I/σ(I)> | 21.1 | 2.4 |
| Completeness [%] | 94.5 | 96.1 |
| Redundancy | 5.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | Hepes, NaCl, NH4OAc, DTT, glycine, PEG 3350, D-ornithine, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
