1SZN
THE STRUCTURE OF ALPHA-GALACTOSIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.489, 79.054, 119.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.540 |
| R-factor | 0.15282 |
| Rwork | 0.151 |
| R-free | 0.18252 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.540 |
| Rmerge | 0.040 | |
| Number of reflections | 65481 | |
| <I/σ(I)> | 12.5 | 1.5 |
| Completeness [%] | 96.1 | 89.3 |
| Redundancy | 7.5 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 6000, potassium phosphate, sodium phosphate , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
