1SZH
Crystal Structure of C. elegans HER-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97931, 0.97917, 0.96866 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.970, 58.930, 99.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.460 - 1.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.460 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 50045 | |
| Completeness [%] | 99.5 | 80.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | PEG 3350, sodium acetate, ammonijm sulfate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
