1SZ9

The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-09
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	56.730, 67.670, 135.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.25200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	ORIGINAL MODEL FROM MAD EXPERIMENT WAS USED FOR FURTHER REFINEMENT. R-FREE SET WAS KEPT CONSISTEND
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	29988
<I/σ(I)>	13.5	4.5
Completeness [%]	98.0	96.7
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	25 % (v/v) ethylene glycol, 5 % (v/v) PEG 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 7.50