1SZ1
Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 218.403, 218.403, 170.263 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.720 - 6.210 |
| R-factor | 0.36131 |
| Rwork | 0.360 |
| R-free | 0.37962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r89 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 6.210 | 6.210 |
| Rmerge | 0.102 | |
| Number of reflections | 10111 | |
| <I/σ(I)> | 12.2 | 1.5 |
| Completeness [%] | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | (NH4)2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | (NH4)2SO4 | ||
| 4 | 1 | 2 | H2O |
