1SYO

N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2000-05-14
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	61.400, 86.400, 86.900
Unit cell angles	90.00, 108.80, 90.00

Refinement procedure

Resolution	29.960 - 2.200
R-factor	0.228
Rwork	0.228
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q25
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.960	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.064	0.301
Number of reflections	38820
Completeness [%]	88.8	55.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.35	292	25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, vapor diffusion, sitting drop, temperature 292K