1SYO
N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-14 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.400, 86.400, 86.900 |
| Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
| Resolution | 29.960 - 2.200 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q25 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.960 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 | 0.301 |
| Number of reflections | 38820 | |
| Completeness [%] | 88.8 | 55.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.35 | 292 | 25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, vapor diffusion, sitting drop, temperature 292K |
