1SY6
Crystal Structure of CD3gammaepsilon Heterodimer in Complex with OKT3 Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.702, 55.770, 96.050 |
| Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 41156 | |
| Completeness [%] | 61.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | PEG3350, potassium formate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 323K, temperature 294K |
