1SXT
STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC
Experimental procedure
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.590, 75.590, 175.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 2.700 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.650 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 * | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 * | 0.227 * |
| Number of reflections | 15899 | |
| Completeness [%] | 91.8 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.25 | 18 * | THE PROTEIN WAS PRE-INCUBATED WITH 0.1 MM ZNSO4. CRYSTALS WAS GROWN USING THE HANGING DROP METHOD FROM 15% PEG 6000, 0.3 M NH4SO4 AND 0.1 M MES BUFFER AT PH 6.25, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 0.05 (mM) | ||
| 3 | 1 | drop | PEG6000 | 7.5 (%(w/v)) | |
| 4 | 1 | drop | ammonium sulfate | 0.15 (M) | |
| 5 | 1 | drop | MES | 0.05 (M) | |
| 6 | 1 | reservoir | PEG6000 | 15 (%(w/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 8 | 1 | reservoir | MES | 0.1 (M) |
