1SX6
Crystal structure of human Glycolipid Transfer protein in lactosylceramide-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-19 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.599, 49.073, 68.538 |
| Unit cell angles | 90.00, 122.52, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.19479 |
| Rwork | 0.192 |
| R-free | 0.24429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.240 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.117 | 0.490 |
| Number of reflections | 16786 | |
| <I/σ(I)> | 15 | 1.7 |
| Completeness [%] | 99.6 | |
| Redundancy | 4.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | PEG 8000, potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
