1SWU
STREPTAVIDIN MUTANT Y43F
Experimental procedure
| Experimental method | MONOCHROMATIC |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.200, 84.900, 46.400 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.140 |
| R-factor | 0.125 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.200 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.056 | 0.144 * |
| Total number of observations | 627749 * | |
| Number of reflections | 149978 | |
| <I/σ(I)> | 13.6 | 5.8 |
| Completeness [%] | 92.0 | 81 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.5 | pH 4.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | 50 (%) |
