1SWP

CORE-STREPTAVIDIN MUTANT W120F IN COMPLEX WITH BIOTIN AT PH 7.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-11-13
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	53.800, 100.000, 51.800
Unit cell angles	90.00, 112.60, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.251
R-free	0.27800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swe
RMSD bond length	0.005
RMSD bond angle	0.019
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.350
Total number of observations	62223 *
Number of reflections	24596
<I/σ(I)>	6.9	1.5
Completeness [%]	90.0	75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	biotin		2.5 molar excess
3	1	reservoir	PEG4000	12-20 (%)
4	1	reservoir	HEPES	0.1 (M)