1SWK

CORE-STREPTAVIDIN MUTANT W79F IN COMPLEX WITH BIOTIN AT PH 4.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-10-30
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	59.300, 64.600, 76.000
Unit cell angles	90.00, 93.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.159 *
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.007
RMSD bond angle	0.025 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.290
Total number of observations	38347 *
Number of reflections	38017
<I/σ(I)>	6.9	2.7
Completeness [%]	97.0	87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	MPD	48 (%)
3	1	drop	biotin		2.5 molar excess