1SWI

GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-08
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	37.210, 46.410, 52.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.187
Rwork	0.187
RMSD bond length	0.019
RMSD bond angle	3.150
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.097
Number of reflections	2618
Completeness [%]	86.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	20 (mg/ml)
2	1	drop	sodium citrate	1.0 (M)
3	1	drop	HEPES	100 (mM)
4	1	reservoir	benzene		0.02 ml to 1 ml of reservoir buffer