1SUM
Crystal structure of a hypothetical protein at 2.0 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9796, 0.9798, 0.9600 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 90.677, 90.677, 45.255 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21993 |
| Rwork | 0.217 |
| R-free | 0.25489 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.196 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14583 | |
| Completeness [%] | 99.0 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM Hepes, 100mM CaCl2, 25% PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
