1STP

STRUCTURAL ORIGINS OF HIGH-AFFINITY BIOTIN BINDING TO STREPTAVIDIN

Experimental procedure

Spacegroup name	I 41 2 2
Unit cell lengths	99.400, 99.400, 125.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.600
R-factor	0.22
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.052 *
Total number of observations	89393 *
Number of reflections	13496 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	9-13 (%(w/v))
2	1	reservoir	citric acid-sodium phsophate		in pH6.0-8.0
3	1	reservoir		1 (M)	can be substituted with 2 M NaCl or KCl