1SSM
Serine Acetyltransferase- Apoenzyme (truncated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2002-05-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.100, 126.600, 107.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 2.150 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.076 | 0.267 |
| Number of reflections | 136283 | |
| Completeness [%] | 0.9 | 76.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM ADA, 1-4% MPD, 190 mM Li2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
