1SRA

STRUCTURE OF A NOVEL EXTRACELLULAR CA2+-BINDING MODULE IN BM-40(SLASH)SPARC(SLASH)OSTEONECTIN

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Detector technology	IMAGE PLATE
Collection date	1995-04-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	54.700, 55.200, 75.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.196
Rwork	0.196
R-free	0.24900
RMSD bond length	0.012
RMSD bond angle	22.700 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.063
Total number of observations	56738 *
Number of reflections	15597
Completeness [%]	98.5
Redundancy	3.6	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	K, Na tartrate	0.7 (M)
2	1	reservoir		2 (mM)