1SQN
Progesterone Receptor Ligand Binding Domain with bound Norethindrone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.753, 64.142, 70.138 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.451 |
| R-factor | 0.18848 |
| Rwork | 0.186 |
| R-free | 0.21586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a28 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.160 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.451 |
| Rmerge | 0.053 |
| Number of reflections | 54571 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 99.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 1000, Li2SO4. hepes pH 6.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
