1SQ9
Structure of Ski8p, a WD repeat protein involved in mRNA degradation and meiotic recombination
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9791, 0.9184, 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.540, 67.640, 80.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.384 |
| Number of reflections | 28833 | |
| <I/σ(I)> | 32 | 5.4 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 8000, ammonium sulfate, cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
