1SQ2

Crystal Structure Analysis of the Nurse Shark New Antigen Receptor (NAR) Variable Domain in Complex With Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1159
Spacegroup name	P 21 21 2
Unit cell lengths	74.020, 109.840, 29.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.450
R-factor	0.19744
Rwork	0.196
R-free	0.22534
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1LKS AND 1QLE (residues 1-85)
RMSD bond length	0.019
RMSD bond angle	1.724
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.480
High resolution limit [Å]	1.450	1.450
Number of reflections	40482
<I/σ(I)>	37.9	2
Completeness [%]	93.1	52.6
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	15% PEG 10,000, 0.1M imidazole HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K