1SPH
REFINED STRUCTURES OF THE ACTIVE S83C AND IMPAIRED S46D HPRS: EVIDENCE THAT PHOSPHORYLATION DOES NOT REQUIRE A BACKBONE CONFORMATIONAL TRANSITION
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.500, 25.700, 60.400 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.640 * |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | NaN * |
| Number of reflections | 9111 |
| Completeness [%] | 92.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salfate | 74-77 (%sat) | |
| 2 | 1 | reservoir | PEG400 | 0.5-1.5 (%(v/v)) | |
| 3 | 1 | reservoir | citrate | 100 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | reservoir |
