1SN8
Crystal structure of the S1 domain of RNase E from E. coli (Pb derivative)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.947390 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.580, 70.580, 87.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.18485 |
Rwork | 0.182 |
R-free | 0.23223 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.417 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 15387 | |
<I/σ(I)> | 17.5 | 2.6 |
Completeness [%] | 98.5 | 86.7 |
Redundancy | 6.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, sodium cacodylate, sodium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |