1SMX

Crystal structure of the S1 domain of RNase E from E. coli (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0781
Spacegroup name	P 41 21 2
Unit cell lengths	70.378, 70.378, 87.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.20009
Rwork	0.199
R-free	0.23109
Structure solution method	SAD
Starting model (for MR)	Pb derivative structure
RMSD bond length	0.013
RMSD bond angle	1.315
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	20912
<I/σ(I)>	30.8	4.65
Completeness [%]	99.0	93.5
Redundancy	7.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	PEG 8000, sodium cacodylate, sodium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K