1SMK

Mature and translocatable forms of glyoxysomal malate dehydrogenase have different activities and stabilities but similar crystal structures

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-10
Detector	CUSTOM-MADE
Wavelength(s)	1.03321
Spacegroup name	P 1 21 1
Unit cell lengths	137.430, 88.050, 138.819
Unit cell angles	90.00, 91.53, 90.00

Refinement procedure

Resolution	13.000 - 2.500
R-factor	0.207
Rwork	0.204
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Partially refinend precursor watermelon glyoxysomal malate dehydrogenase
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.660
High resolution limit [Å]	2.500	2.500
Number of reflections	98023
Completeness [%]	85.3	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	PEG 8000, Sodium Citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K