1SMH
Protein kinase A variant complex with completely ordered N-terminal helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.780, 79.431, 117.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.044 |
| R-factor | 0.19076 |
| Rwork | 0.188 |
| R-free | 0.23789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.426 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.100 |
| High resolution limit [Å] | 2.044 | 2.044 |
| Number of reflections | 29113 | |
| Completeness [%] | 97.8 | 91.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 278 | LiCl, MesBisTris, methanol, MEGA-8, (R,R)-2,3-butanediol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
