Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9205, 0.8434, 0.9207 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.500, 53.850, 75.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| Rwork | 0.198 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 7865 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | MgCl2, HEPES-Na, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | HEPES-Na | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 2 | PEG 400 |
