1SL4

Crystal Structure of DC-SIGN carbohydrate recognition domain complexed with Man4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-13
Detector	ADSC
Wavelength(s)	0.97949
Spacegroup name	P 43
Unit cell lengths	55.660, 55.660, 53.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 1.550
Rwork	0.223
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.450	1.590
High resolution limit [Å]	1.550	1.550
Number of reflections	23776
Completeness [%]	100.0	99.8
Redundancy	9.9	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG 400, 0.1M Tris pH 7.0.,Protein solution: 10 mg/ml protein, 5mM CaCl2, 10 mM oligosaccharide., VAPOR DIFFUSION, HANGING DROP, temperature 298K