1SJS
ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING
Experimental procedure
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1994-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 124.500, 77.500, 92.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.420 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.24800 * |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.420 |
| Rmerge | 0.075 |
| Total number of observations | 37773 * |
| Number of reflections | 13753 * |
| Completeness [%] | 69.0 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 11 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | HEPES | 100 (mM) | |
| 4 | 1 | drop | ATP gamma S | 0.05 (M) | |
| 5 | 1 | drop | 0.05 (M) | ||
| 6 | 1 | drop | dithiothreitol | 2 (mM) | |
| 7 | 1 | drop | 0.01 (%) | ||
| 8 | 1 | reservoir | PEG4000 | 29-32 (%) | |
| 9 | 1 | reservoir | ammonium acetate | 10-90 (mM) |
