1SJC
x-ray structure of o-succinylbenzoate synthase complexed with N-succinyl methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-07 |
| Wavelength(s) | 0.6525 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 215.700, 215.700, 259.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 154522 | |
| <I/σ(I)> | 37.6 | 3 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 9.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 298 | PEG 8000, HEPES, MgCl2, n-succinylmethionine, pH 8.0, batch, temperature 298K |
