1SIE
MURINE POLYOMAVIRUS COMPLEXED WITH A DISIALYLATED OLIGOSACCHARIDE
Experimental procedure
| Spacegroup name | I 2 3 |
| Unit cell lengths | 570.000, 570.000, 570.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.650 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.26400 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 3.650 |
| Number of reflections | 244414 |
| Completeness [%] | 74.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 6-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | sodium sulfate | 0.25-0.3 (M) | |
| 4 | 1 | drop | glycerol | 2.5-5 (%(v/v)) | |
| 5 | 1 | reservoir | sodium sulfate | 0.55-0.6 (M) | |
| 6 | 1 | reservoir | HEPES | 10 (mM) | |
| 7 | 1 | reservoir | glycerol | 5-10 (%) |
