1SI3

Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-18
Detector	MARRESEARCH
Wavelength(s)	0.9796
Spacegroup name	P 64
Unit cell lengths	99.799, 99.799, 34.933
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.830 - 2.600
R-factor	0.247
Rwork	0.247
R-free	0.30400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	6431
<I/σ(I)>	28.6	3.4
Completeness [%]	99.9	100
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	PEG 1000, POTASSIUM CHLORIDE, HEPES-NAOH, DITHIOTHREITOL, pH 7.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 1000
2	1	1	POTASSIUM CHLORIDE
3	1	1	HEPES-NAOH
4	1	1	DITHIOTHREITOL