1SHX
Ephrin A5 ligand structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.210, 70.080, 58.460 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| Rwork | 0.224 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EprinB2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | |
| Number of reflections | 16487 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 91.3 | 54.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
