1SHQ

Crystal structure of shrimp alkaline phosphatase with magnesium in M3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2002-05-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2825
Spacegroup name	P 43 21 2
Unit cell lengths	171.007, 171.007, 84.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.530 - 2.000
R-factor	0.21645
Rwork	0.215
R-free	0.23808
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.026
RMSD bond angle	1.928
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	42% (w/v) saturated ammonium sulfate, 100mM tris-maleic acid pH 5.6, 100 mM MgCl2, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K