1SHN

Crystal structure of shrimp alkaline phosphatase with phosphate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	77
Detector technology	CCD
Collection date	2002-02-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 43 21 2
Unit cell lengths	170.676, 170.677, 83.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.640 - 2.150
R-factor	0.18758
Rwork	0.186
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.969
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.150
Number of reflections	66456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	42% (w/v) saturated ammonium sulfate, 100mM tris-maleic acid pH 5.6, 1 mM MgCl2, 0.1mM ZnCl2, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K