1SGW

Putative ABC transporter (ATP-binding protein) from Pyrococcus furiosus Pfu-867808-001

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-25
Detector	MARRESEARCH
Wavelength(s)	1.066
Spacegroup name	P 21 21 21
Unit cell lengths	44.982, 67.583, 72.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.1968
Rwork	0.195
R-free	0.22299
RMSD bond length	0.011
RMSD bond angle	1.294
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge		0.123
Number of reflections	23651
Completeness [%]	99.4	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	modified microbatch	7.9	291	15% PEG-4000, 10% isopropanol, 10% glycerol, 100mM TRIS, pH 7.9, modified microbatch, temperature 291K