1SGL
The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.990, 93.990, 57.765 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.560 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.560 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 14868 | |
| <I/σ(I)> | 4609.6 | 575.1 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, tris-hydrochloric acid, potassium hydrogen sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
