1SGF
CRYSTAL STRUCTURE OF 7S NGF: A COMPLEX OF NERVE GROWTH FACTOR WITH FOUR BINDING PROTEINS (SERINE PROTEINASES)
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG |
Synchrotron site | EMBL/DESY, HAMBURG |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1990-05 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.680, 96.590, 147.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.150 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BETA-NGF AND PORCINE PANCREATIC KALLIKREIN (1BET AND 2PKA) |
RMSD bond length | 0.008 |
RMSD bond angle | 27.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | TFFC |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.180 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.065 | 0.427 |
Number of reflections | 24209 | |
<I/σ(I)> | 10.5 | 1.7 |
Completeness [%] | 96.0 | 92.5 |
Redundancy | 2.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.8 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 20MG/ML PROTEIN SOLUTION IN 50MM SODIUM PHOSPHATE (PH6.8) MIXED WITH AN EQUAL VOLUME OF WELL SOLUTION (50MM SODIUM ACETATE PH4.5-5.1, 12-21% PEG 4000, 10 MICROMOLAR ZNSO4) (MCDONALD AND BLUNDELL, 1991, JMB, 219, 595-601)., pH 4.9 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 50 (mM) | |
3 | 1 | reservoir | sodium acetate | 50 (mM) | |
4 | 1 | reservoir | 0.01 (M) | ||
5 | 1 | reservoir | PEG4000 | 12-16 (%(w/v)) |