1SGF
CRYSTAL STRUCTURE OF 7S NGF: A COMPLEX OF NERVE GROWTH FACTOR WITH FOUR BINDING PROTEINS (SERINE PROTEINASES)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1990-05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.680, 96.590, 147.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.150 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BETA-NGF AND PORCINE PANCREATIC KALLIKREIN (1BET AND 2PKA) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | TFFC |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.065 | 0.427 |
| Number of reflections | 24209 | |
| <I/σ(I)> | 10.5 | 1.7 |
| Completeness [%] | 96.0 | 92.5 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 20MG/ML PROTEIN SOLUTION IN 50MM SODIUM PHOSPHATE (PH6.8) MIXED WITH AN EQUAL VOLUME OF WELL SOLUTION (50MM SODIUM ACETATE PH4.5-5.1, 12-21% PEG 4000, 10 MICROMOLAR ZNSO4) (MCDONALD AND BLUNDELL, 1991, JMB, 219, 595-601)., pH 4.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 4 | 1 | reservoir | 0.01 (M) | ||
| 5 | 1 | reservoir | PEG4000 | 12-16 (%(w/v)) |
