1SFT

ALANINE RACEMASE

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-11-26
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	98.590, 90.130, 85.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.193
Rwork	0.193
R-free	0.26700
RMSD bond length	0.019
RMSD bond angle	26.050 *
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.900
Rmerge	0.046
Number of reflections	52379
<I/σ(I)>	9.3
Completeness [%]	83.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	PEG4000	30 (%(w/v))
3	1	drop	sodium acetate	200 (mM)
4	1	drop	Tris-HCl	100 (mM)
5	1	reservoir	PEG4000	30 (%(w/v))
6	1	reservoir	sodium acetate	200 (mM)
7	1	reservoir	Tris-HCl	100 (mM)