1SFE

ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Detector	WEISSENBERG
Spacegroup name	P 1 21 1
Unit cell lengths	46.200, 45.950, 46.400
Unit cell angles	90.00, 114.06, 90.00

Refinement procedure

Resolution	39.000 - 2.100
R-factor	0.219 *
Rwork	0.219
RMSD bond length	0.010
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.058
Total number of observations	19437 *
Number of reflections	9017
Completeness [%]	86.0
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *
1	Microdialysis *
1	Microdialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5-50 (mg/ml)
2	1	1	sodium acetate	200-500 (mM)
3	1	1	dithiothreitol	10 (mM)
4	1	1	Tris-acetate	10-20 (mM)
5	1	2	dithiothreitol	10 (mM)
6	1	2	glucose	300 (mM)	can be replaced by maltose
7	1	2	Tris-acetate	10 (mM)