1SFC
NEURONAL SYNAPTIC FUSION COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-05-15 |
| Detector | ADSC |
| Wavelength(s) | 1.0332,0.9797,1.0688,0.9801,0.9795,0.9800 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.715, 111.071, 198.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.30300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 * | 0.329 * |
| Total number of observations | 309408 * | |
| Number of reflections | 42980 | |
| <I/σ(I)> | 17.4 | 7.6 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 29 * | 35% MPD, 5% PEG350(MME), 25MM TRIS PH 7.0,. 70MM SRCL2, 250MM UREA, 7MM SARKOSYL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 35 (%(v/v)) | |
| 10 | 1 | drop | urea | 175 (mM) | |
| 11 | 1 | drop | Sarkosyl | 3.75 (mM) | |
| 12 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG350 MME | 5 (%(v/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 25 (mM) | |
| 4 | 1 | reservoir | 70 (mM) | ||
| 5 | 1 | reservoir | urea | 250 (mM) | |
| 6 | 1 | drop | MPD | 24.5 (%(v/v)) | |
| 7 | 1 | drop | PEG350 MME | 3.5 (%(v/v)) | |
| 8 | 1 | drop | Tris | 17.5 (mM) | |
| 9 | 1 | drop | 49 (mM) |
