1SF8
Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1271, 0.9791, 0.9790 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 103.516, 103.516, 249.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.600 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.630 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 79708 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 96.3 | 70.5 |
| Redundancy | 5.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium malonate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
