1SDV
Crystal structures of HIV protease V82A and L90M mutants reveal changes in indinavir binding site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-06-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.820, 58.726, 46.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.1597 |
| Rwork | 0.160 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1daz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Completeness [%] | 88.7 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | Citrate/phosphate buffer, NaCl , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
