1SDO

Crystal Structure of Restriction Endonuclease BstYI

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-01
Detector	SBC-2
Wavelength(s)	0.973, 0.9282
Spacegroup name	I 2 2 2
Unit cell lengths	69.890, 76.610, 78.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
Rwork	0.220
R-free	0.24100
Structure solution method	MAD
RMSD bond angle	0.007
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Rmerge	0.092
Number of reflections	33604
<I/σ(I)>	17.4
Completeness [%]	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30% PEG-400, 0.1M HEPES, 200mM Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K