1SDJ

X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.

Replaces:  1P9V

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97929, 0.97970, 0.97702
Spacegroup name	C 2 2 21
Unit cell lengths	125.047, 130.659, 48.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.218
Rwork	0.218
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.075	0.247
Number of reflections	32871
Completeness [%]	89.5 *	85.6
Redundancy	4.3	27 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ligand	10 (mM)
3	1	reservoir	PEG4000	24-30 (%(w/v))
4	1	reservoir	sodium citrate	0.1 (M)	pH4.4-5.0
5	1	reservoir		0.2 (M)